G3057-20A
Glucose Regulated Protein 78 (Grp78, BiP, GRP 78)
Size
£620.00
- SKU:
- G3057-20A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa643-654 of rat GRP78 BiP (C-TGEEDTSEKDEL).
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol
- Extra Details:
- Within the ER misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphorylate. In its dimerized, phosphorylated form PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. Bip also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up-regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml Immunofluorescence (IC-IF): 1:20 Optimal dilutions to be determined by the researcher. Positive Control: Rat liver extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice