G2100-01

Glucoamylase (Biotin)

Size

£889.00

SKU:: G2100-01
Purity:: Purified by delipidation, salt fractionation and ion exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Glucoamylase (Rhizopus)
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul sterile dH2O. Labeled with Biotin.
Extra Details:: Glucoamylase is an enzyme preparation which decomposes starch into glucose by tearing-off glucose units from the non-reduced end of the polysaccharide chain. It is derived by submerged fermentation of a specially selected producer strain of Asp. niger. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:75,000; 1ug of Glucoamylase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT. Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.