Glucocorticoid Receptor alpha

Size

£677.00

SKU:: G2040-52
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to amino acids 755-771 [CEIITNQIPKYSNGNIKK] of human Glucocorticoid Receptor alpha.
Format:: Supplied as a lyophilized powder in 0.05% sodium azide. Reconstitute with sterile PBS.
Extra Details:: Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the a and b forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94kD GRalpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90kD GRbeta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GRα has a greater affinity for GREs than GRbeta only when in the ligand bound state. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 4ug/ml, detects a band of ~90kD representing GR in HeLa cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice