G2040-39D
Glucagon-like Peptide 2 Receptor (GLP2R)
Size
£825.00
- SKU:
- G2040-39D
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HEK293 human embryonic kidney cell line transfected with human GLP-2R (Met1-Ile553).
- Clone:
- 7H244
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- GLP2R is a member of the class II glucagon-secretin G protein-coupled receptor superfamily. It is expressed on enteroendocrine cells in the gut where it mediates the positive effects of GLP2 on villus height, crypt depth and intestinal energy absorbtion. After stimulating a cAMP response, lipid raft-dependent internalization of GLP2R results in rapid desensitization to GLP2. Extracellular portions of human GLP2R show approximately 80% amino acid identity with corresponding regions of mouse GLP2R. Applications: Suitable for use in Immunocytochemistry, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml. Immersion fixed HeLa human cervical epithelial carcinoma cell line Flow Cytometry: 2.5ug/10e6 cells. NS0 mouse myeloma cell line transfected with human GLP-2R. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice