G2040-35C

Glucagon-like Peptide 1 (GLP1)

Size

£345.00

SKU:: G2040-35C
Purity:: Purified by Protein G affinity chromatography from cell culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa7-36 of human GLP-1 conjugated to KLH.
Clone:: GLP-1/18
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 10ul or 100ul sterile ddH2O.
Extra Details:: GLP1, a processed active peptide of 30aa (chr 2q36-q37) is a potent insulin secretagogue, and plays a major role in the enteroinsular axis, accounting for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously. Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulate intestinal growth and up regulate villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration. Applications: Suitable for use in Electron Microscopy, Dot Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:80-1:8000 Electron Microscopy: 1:600 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice