G2040-01K

Glucagon (BSA, Azide, Glycerol Free)

Size

£774.00

SKU:: G2040-01K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 8aa in the middle region of human Glucagon conjugated to KLH.
Format:: Supplied as a liquid in PBS, also available with BSA, see G2040-01H.
Extra Details:: Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration. Glucagon (GLGN) is a 29aa pancreatic hormone (chr 2q36), which together with insulin, is an important regulator of glucose metabolism. When in need of glucose, glucagon is secreted by the A cells of the pancreas and binds to target cell-surface receptors in the liver. This triggers a series of events that ultimately results in glycogenolysis and gluconeogenesis and a consequent rise in glucose levels in the blood. The glucagons in Human, rabbit, rat, porcine and bovine are identical. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml (ECL) ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well (See G2040-01J). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice