Glial Derived Neurotrophic Factor (GDNF) (Biotin)

Size

£479.00

SKU:: G2036-05A
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant human Glial Derived Neurotrophic Factor (GDNF), expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with sterile PBS containing 0.1% BSA.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Using 100ul/well antibody solution, a concentration of 0.15-0.3ug/ml of this antibody is required. When used in conjunction with with G2036-01A as capture antibody, allows the detection of 0.2ng/well of recombinant hGDNF. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDNF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.