Glial Derived Neurotrophic Factor (GDNF)

Size

£572.00

SKU:: G2036-01A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant human GDNF, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute in 100ul sterile ddH2O.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with G2036-05A as a detection antibody, allows the detection of at least 0.2-0.4n/ml of recombinant human GDNF Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human GDNF is 1.5-3ug/lane under non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.