G2035-08W

GITR (Glucocorticoid-induced TNFR-related Protein, Activation-inducible TNFR Family Receptor, AITR, CD357, Tumor Necrosis Factor Receptor Superfamily Member 18, TNFRSF18, UNQ319/PRO364) (PE)

Size

£735.00

SKU:: G2035-08W
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human GITR and PHA-stimulated human PBL
Clone:: 11K8
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide and 15% glycerol. Labeled with R-Phycoerythrin (PE).
Extra Details:: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3). Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50; 1.6ul of concentrated G2035-08W labels 5x10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry: Ficoll prepared human peripheral blood leukocytes (PBL) were stimulated 5 days in culture with PHA. 1. 5x10e5 cells per tube were washed and pre-incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80ul of G2035-08W at a 1:50 dilution (10ug/ml). 2. Cells were washed three times and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a mouse IgG3-R-PE negative control at a similar concentration. 3. Binding was blocked when cells were pre-incubated with a 10-fold excess of G2035-08S (unlabeled anti-GITR mAb). Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice