Gi alpha 3 (Not for Export EU)

Size

£691.00

SKU:: G2033-91
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 10aa, KNNLKECGLY from the C-terminal region of Gi alpha 3.
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: Gi alpha 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-gamma activation and Ca+2 mobilization in hepatocytes1,2. G-proteins are membrane associated heterotrimeric proteins that are comprised of alpha, beta-, and lambda-subunits. The alpha-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the alpha-subunit from the beta, lambda-subunit complex. This enables the G alpha-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the alpha-subunit with the beta, lambda- subunit complex. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 detected Gi alpha 3 in rat hippocampus membrane preparation. Previous lots detected Gi apha 3 in solubilized rat and mouse brain membranes and mouse 3T3/A31 cell lysates. Immunoprecipitation: 4ul immunoprecipitated Gi alpha 3 from a solubilized mouse brain membrane lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice