Gi alpha 1,2

Size

£767.00

SKU:: G2033-90
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide (KNNLKDCGLF) corresponding to the C-terminal region shared by rat Gia-1 and Gia-2, coupled to KLH.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added.
Extra Details:: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone. Optimal dilution determined by the researcher. Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice