G2033-75E

Ghrelin

Size

£754.00

SKU:: G2033-75E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 13aa (n-octanoylated at Ser3) from the N-terminus of mature human ghrelin, conjugated to KLH. Species Sequence Homology: porcine: (94%); bovine, canine, mouse and rat: (84%). Des-ghrelins have the same sequence.
Format:: Supplied as a liquid in PBS, 0.1 BSA.
Extra Details:: Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117aa precursor. In rat stomach, a 28aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid. Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml using ECL. Full length ghrelin is ~3.5kD. ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well. Immunohistochemistry: 10-30ug/ml in formaldehyde fixed, paraffin embedded tissues. Optimal dilutions to be determined by the researcher. Control Peptide: G2033-75F: Ghrelin, Human Control Peptide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice