G2024-52

Gelsolin (Actin depolymerizing factor, ADF, AGEL, Amyloidosis Finnish type, Brevin, GSN, Calcium regulated actin binding protein)

Size

£1,077.00

SKU:: G2024-52
Purity:: Purified by Protein A/G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Gelsolin purified from human plasma.
Clone:: 9j134 (20)
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions. Applications: Suitable for use in Western Blot Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:6000 Immunofluorescence: 1:2000 Note: Does not work as capture antibody in sandwich ELISA. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.