G2023-55
GCP 2 (Chemokine (CXC motif) ligand 6, Chemokine alpha 3, CKA3, CXCL6, Granulocyte chemotactic protein 2, SCYB6, Small inducible cytokine subfamily B (Cys X Cys) member 6)
Size
£442.00
- SKU:
- G2023-55
- Purity:
- Purified by Immunoaffinity chromatography. Endotoxin: <0.1ng/ug protein (<1EU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human GCP 2 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Human GCP-2 is an 8kD protein containing 73 amino acid residues. Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP2 (CXCL6). GCP2 belongs to the intercrine alpha (chemokine CxC) family. GCP-2 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilution: Neutralization: 2-5ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of human GCP 2 (100ng/ml). ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with biotylated anti-human GCP 2 (G2023-55A) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human GCP 2. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human GCP 2 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice