G2023-24

GCET2 (Germinal Center B Cell-expressed Transcript 2 Protein, Germinal Center-associated Lymphoma Protein, hGAL, GAL)

Size

£685.00

SKU:: G2023-24
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant protein corresponding to aa1-179 from human GCET2 with GST tag (AAH30506). MW of the GST tag alone is 26kD.
Clone:: 1E9
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: GCET2, commonly known as germinal center B cell expressed transcript 2 protein, is a 178aa protein expressed in diffuse large B cell lymphoma (DLBCL) and several germinal center (GC) like lymphoma cell lines (at protein level). It is highly expressed in normal GC lymphocytes and GC derived malignancies, as well as being present in the thymus and spleen. In B cells expression of the HGAL gene is specifically induced by interleukin 4. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1-5ug/ml using recombinant protein. Western Blot (Cell lysate): 1-5ug/ml. Specific band identified at ~21kD using Jurkat lysate. Western Blot (Recombinant lysate protein): 1-5ug/ml. Specific band of ~45kD identified using immuogen protein lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.