G beta (GNB2, SG, IG)

Size

£767.00

SKU:: G2022
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Internal sequence of G-protein beta subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added.
Extra Details:: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 detects the G protein beta subunits in 20ug of a cell lysate prepared from rat brain microsomal preparation. Immunoprecipitation: 10ul immunoprecipitates the G protein beta subunit from 0.5-1mg of a lysate prepared from rat brain microsomal preparation. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice