G2021-40B
GATA-2 (GATA binding protein 2, Endothelial transcription factor GATA-2, FLJ45948, MGC2306, NFE1B)
Size
£832.00
- SKU:
- G2021-40B
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala15-Thr279 from human GATA-2, expressed in E. coli.
- Clone:
- 9H127
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues. Specifically, GATA-2 is expressed in hematopoietic progenitors and stem cells. GATA-2 expression is associated with cell proliferation. GATA-2 is 480 amino acids in length and contains 2 Zn finger domains responsible for DNA binding (aa295-319 and aa349-373). Over aa15-279 human GATA-2 share 96% identity with mouse GATA-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml with K562 human chronic myelogenous leukemia cell line and DA3 mouse myeloma cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice