G2010-10C
GAPDHS (Glyceraldehyde-3-phosphate Dehydrogenase, Testis-specific, Spermatogenic Cell-specific Glyceraldehyde 3-phosphate Dehydrogenase 2, GAPDH-2, Spermatogenic Glyceraldehyde-3-phosphate Dehydrogenase, GAPD2, GAPDH2, GAPDS, HSD35, HSD-35)
Size
£837.00
- SKU:
- G2010-10C
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to Glu75-Ala222 from human GAPDHS expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- GAPDS is a 40-44kD member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56kD in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408aa in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa223-226. One splice variant shows a 9aa insertion after Ser220. Over aa75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice