G2005-66-PE

Ganglioside GD3 (PE)

Size

£1,023.00

SKU:: G2005-66-PE
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: FM9 melanoma cells.
Clone:: 2Q631
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Extra Details:: GD3 has been identified as tumor-associated antigens expressed on the surface of neuroectoderm-derived tumors (melanoma, neuroblastoma and glioma) and T-cell leukemia. Gangliosides (sialic acid-containing glycosphingolipids) are normal membrane constituents and highly expressed in the vertebral central nervous system. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen tissue sections Immunofluorescence: Cells in culture Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.