G2005-66-BIOTIN

Ganglioside GD3 (Biotin)

Size

£1,023.00

SKU:: G2005-66-BIOTIN
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: FM9 melanoma cells.
Clone:: 2Q631
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: GD3 has been identified as tumor-associated antigens expressed on the surface of neuroectoderm-derived tumors (melanoma, neuroblastoma and glioma) and T-cell leukemia. Gangliosides (sialic acid-containing glycosphingolipids) are normal membrane constituents and highly expressed in the vertebral central nervous system. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen tissue sections Immunofluorescence: Cells in culture Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.