G1044-71
Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13)
Size
£780.00
- SKU:
- G1044-71
- Application:
- WB
- Purity:
- Purified by Protein G immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14; aa 1-139; Accession# Q8TCE9).
- Clone:
- 9H126
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20. Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice