G1044-50M

Galectin-3, ID (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein, Lectin Galactose Binding Soluble 3, LGALS3, MAC2)

Size

£735.00

SKU:: G1044-50M
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human Galectin 3.
Format:: Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: Galectin-3, a 29-31kD protein, is a member of large family of b-galactoside binding animal lectins. It is a chimeric molecule consisting of both carbohydrate recognition and collagen-like domains, with an extra N-terminal tail consisting of 8-13 copies of a 9aa repeat consensus sequence that is rich in proline, tyrosine, and glycine. It is mainly a cytosolic molecule but can easily traverse the intracellular and the plasma membranes to visit the nucleus, mitochondria. It is secreted by various cell types including monocytes, macrophages, and epithelial cells. The released protein can function as an extracellular molecule to activate cells, mediate cell-cell and cell-ECM interactions, and induce migration of monocytes, macrophages, and endothelial cells, probably by binding to pertinent receptors through lectin-carbohydrate interactions. More recently, cell surface galectin-3 was implicated in restricting T cell receptor clustering and thus negatively regulating T cell activation. As a multifunctional protein it acts as an antiapoptotic protein and protects cells from apoptosis by inducing G1 arrest in response to loss of cell anchorage. Galectin-3 has also been associated with the progression of cancer, as well as being a mediator of inflammation. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000 Western Blot: 1:500-1:1000 Immunofluorescence: 1:100-1:500 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice