G1044-50D1

Galectin-3 (Galactose-specific Lectin 3, Galectin 3, GAL3, Gal-3, 35kD Lectin, Carbohydrate Binding Protein 35, CBP 35, CBP35, Galactoside Binding Protein, GALBP, GALIG, IgE Binding Protein, L-31, Laminin Binding Protein, Lectin Galactoside Binding Soluble 3, LGALS3, Lectin L-29, MAC2, Mac-2 Antigen) (Biotin)

Size

£629.00

SKU:: G1044-50D1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human galectin-3, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile ddH2O contailing 0.1% BSA.
Extra Details:: Galectin-3 (Gal-3), is a member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties. Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumor metastasis, and several studies have shown a correlation between levels of Gal-3 and tumor progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: To detect by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required. In conjunction with G1044-50A as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hGalectin-3. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice