G1044-50A-PE

Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein, Lectin Galactose Binding Soluble 3, LGALS3, MAC2) (PE)

Size

£812.00

SKU:: G1044-50A-PE
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Galectin-3, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Extra Details:: Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Galectin 3 is one of the more extensively studied member of this family and is a 30kD protein known by various other names including eBP (IgE binding protein), Mac-2, CBP-30 and L-29. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. This antibody has been shown to potentiate the binding of Galectin 3 to IgG. Among its numerous proposed functions, Galectin 3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin 3 shares an NWGR motif with Bcl-2 which has been shown to be an important region in Bcl-2 for suppression of apoptosis. Galectin 3 has been shown to interact with Bcl-2 which may account for the ability of Galectin 3 to suppress programmed cell death in anti-Fas (an apoptosis inducer) treated Jurkat cells transfected with Galectin 3. In contrast to Galectin 3, Galectin 1 actually induces apoptosis when added to cultures of activated human T cells. Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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