G1042-40

GGTB2 (Beta-1,4-galactosyltransferase 1, B4GALT1, Beta4Gal-T1, UDP-Gal:beta-GlcNAc, N-acetyllactosamine Synthase, Nal Synthase, Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase)

Size

£832.00

SKU:: G1042-40
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gly44-Ser398 luminal domain of human B4GalT1 expressed in NSO cells. Species Sequence Homology: rat (88%); mouse (87%); canine (87%); chicken (71%).
Clone:: 7H199
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Beta-1, 4-galactosyltransferase 1 (B4GalT1) is one of seven B4GalT genes. Human B4GalT1 encodes a type II membrane protein, which is ubiquitously expressed and involved in glycoconjugate biosynthesis. A soluble form (aa78-398), derived from proteolytic cleavage, is a component of lactose synthase in milk that also contains alpha-lactalbumin. The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken. Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml The detection limit for recombinant human B4GaIT1 is ~5ng/lane under non-reducing and reducing conditions using a colormetric detection system. Chemiluminescent detection will increase sensitivity by 5-50 fold Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human B4GaIT1 from conditioned cell culture medium. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice