G1041-44C

β-Galactosidase (Beta-galactosidase, Galactosidase beta 1, GLB1, Acid beta-galactosidase, EBP, Elastin Receptor 1, ELNR1, Lactase, MPS4B)

Size

£698.00

SKU:: G1041-44C
Purity:: Purified by Ion exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Beta galactosidase prepared from E. coli.
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Beta galactosidase is an inducible enzyme that catalyses the hydrolysis of lactose and other betagalactosides into monosaccharides. Beta galactosidase is a product of the LacZ operon, and is a homotetrameric protein consisting of four identical subunits of ~116kD each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 1:4000-1:1600 Immunohistochemistry (frozen and paraffin): 1:50-1:300 Western Blot: 1:200-1:500, detects a band of ~117kD using E. coli beta-galactosidase. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.