G1041-05E
β-Galactosidase (E. coli) (HRP)
Size
£704.00
- SKU:
- G1041-05E
- Purity:
- Purified by ion exchange chromatography prior to conjugation.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant purified E. coli b-Galactosidase. (~16kD)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, before the addition of glycerol to 40%. Labeled with HRP.
- Extra Details:
- Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3). Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation. Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6). Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Antibody HRP Molar Ratio: ~1.0-1.5 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice