G1041-05B
beta-Galactosidase (E. coli)
Size
£863.00
- SKU:
- G1041-05B
- Purity:
- Purified by ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant purified E. coli b-Galactosidase. (~16kD)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3). Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation. Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6). Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice