G1016-ML650
GABA A Receptor beta 2,3 (MaxLight 650)
Size
£1,133.00
- SKU:
- G1016-ML650
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Affinity-purified GABAA receptor isolated from bovine brain.
- Clone:
- 0.T.52 (62-3G1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detected the GABA A Receptor beta 2,3 subunits from 20ug of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G1016 (2ug/ml). Proteins were visualized using a IgG (HRP) GtxMo and ECL. Immunoprecipitation: 4ug immunoprecipated the GABA A Receptor beta 2,3 subunits from 500ug of rat brain microsomal preparation. Due to interference of the IgG heavy chain, the immunoprecipitated GABA A reseptor complex was confirmed using G1015-06. Immunohistochemistry: Detects GABA A Receptor beta 2,3 subunits in paraffin embedded and frozen rat brain sections fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice