G1016-50

GAL4

Size

£456.00

SKU:: G1016-50
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-147 mapping within the N-terminal, DNA binding domain of GAL4.
Clone:: 3G152
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: GAL4 protein is a transcriptional activator that is required for the expression of genes that encode enzymes of galactose utilization. The N-terminal domain of GAL4 binds to specific DNA sequences and the C-terminal domain contains acidic regions, which are required for activating transcription. GAL4 has been useful in two-hybrid assays for the study of protein-protein interactions. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunoprecipitation, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.