G1016-50-FITC
GAL4 (FITC)
Size
£947.00
- SKU:
- G1016-50-FITC
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-147 mapping within the N-terminal, DNA binding domain of GAL4.
- Clone:
- 3G152
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- GAL4 protein is a transcriptional activator that is required for the expression of genes that encode enzymes of galactose utilization. The N-terminal domain of GAL4 binds to specific DNA sequences and the C-terminal domain contains acidic regions, which are required for activating transcription. GAL4 has been useful in two-hybrid assays for the study of protein-protein interactions. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Immunoprecipitation, FLISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice