G1016-50-APC

GAL4 (APC)

Size

£947.00

SKU:: G1016-50-APC
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-147 mapping within the N-terminal, DNA binding domain of GAL4.
Clone:: 3G152
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: GAL4 protein is a transcriptional activator that is required for the expression of genes that encode enzymes of galactose utilization. The N-terminal domain of GAL4 binds to specific DNA sequences and the C-terminal domain contains acidic regions, which are required for activating transcription. GAL4 has been useful in two-hybrid assays for the study of protein-protein interactions. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Immunoprecipitation, FLISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.