GABA Receptor B R2 (Gamma-aminobutyric Acid Receptor Type B)

Size

£767.00

SKU:: G1015-50T
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 24aa peptide of rat GBR2, conjugated to KLH. Location: ~C-terminus, Cytoplasmic domain. Species Sequence Homology: Canine, chimpanzee, human, mouse - 100%; Chicken - 91%. No significant homology is detected with GBR1 or other receptors.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain. GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs). Presynaptic GABABRs inhibit neurotransmitter release by down regulating high voltage activated, Ca+2 channels, whereas postsynaptic GABABRs decrease neuronal excitability by activating a prominent inwardly rectifying K+ (Kir) conductance that underlies the late inhibitory postsynaptic potential. Two N-terminal splice variants of GABABR1 (also called GBR1), GABABR1a (GBR1a, rat 960aa, apparent mol wt ~130kD; human 961aa) and GABABR1b (GBR1b; rat 844aa, apparent mol. wt 100kD; human 844aa) have been cloned and characterized. Human and rat GBR1a and GBR1b share 99% sequence homology. GABABRs are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 7 TM domains, and C-terminal, cytoplasmic domain. GABABRs are expressed in all major areas of the brain. Recently GABABR2 (or GBR2) has been cloned from rat (940aa) and human (941aa). GBR2 forms heterodimer with GBR1 through interaction at the intracellular C-terminal regions. Heterodimerization appears to be important in transporting GBR1 to the membrane and expression of functional receptors. GBR2 also colocalized with GBR1. Applications: Western Blot: 1-10ug/ml using ECL technique. ELISA: 0.5-1ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice