GABA Receptor Associated Protein (GABARAP)

Size

£767.00

SKU:: G1015-24A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 19 AA Peptide sequence near the N-terminus of human GABARAP (2). The peptide was coupled to KLH
Format:: supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Extra Details:: GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain. GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs). A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric) protein complexes. Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1). GABA A (GAA) receptor proteins (450-627 aa) are characterized by the presence of a large cytoplasmic domain that may be involved in anchoring the receptor to the cytoskeleton. Most recently, a new cellular protein, termed GABA A receptor associated protein (GABARAP), has bee shown to interact with gamma 2 subunit of GAA. GABARAP has similarity with light chain-3 microtubule associated protein 1A and 1B. N-terminus of GABARAP also possess a tubulin-binding motif. Therefore, a possible interaction of GAA, GABARAP, and tubulin suggest for the targeting and clustering of GAA. GABARAP is found in brain and most other tissues. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting (1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. ELISA Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice