GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA1) (FITC)

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£1,151.00

SKU:: G1015-06-FITC
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to the topological domain of rat GABA Receptor A, alpha1, conjugated to KLH. Species Sequence Homology: human, 94%
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: The neurotransmitter GABA binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. The GABAA receptor is a multiprotein complex that acts as a ligand-gated chloride channel. There are at least 6 different isoforms of the alpha subunit. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: detects the GABA Receptor A, alpha1 subunit in 10ug of rat brain microsomal preparation. tissue lysate. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 5ul immunoprecipitates the GABA Receptor A, alpha1 subunit in rat brain microsomal preparation RIPA lysate. Immunohistochemistry (Paraffin): detects the GABA Receptor A, alpha1 subunit in rat hippocampus tissue. Requires HIER. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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