F9500-08-ML650
Fyn, p59 (MaxLight 650)
Size
£927.00
- SKU:
- F9500-08-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- A fusion protein consisting of aa1-206 of murine p59 fyn.
- Clone:
- 3G151
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Fyn is a 59kD non-receptor protein tyrosine kinase belonging to the Src family of cytoplasmic tyrosine kinases. Members of this family possess a similar structural organization which can be divided into four distinct domains including: 1) N-terminal myristoylation on glycine- required for membrane localization, 2) a unique N-terminal domain which may dictate specificity of association, 3) conserved SH3 and SH2 domains, and 4) a C-terminal catalytic domain(2). Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing(3). Fyn was also the first tyrosine kinase found to be associated with the T cell antigen receptor complex(4). The interaction of Fyn with the TCR complex is mediated by the amino terminal domain of Fyn and at least one ARAM sequence (antigen recognition activation motif) in the TCR zeta chain (TCRz)(2). In addition to its association with TCRz, Fyn has been found to associate with the p21ras interacting protein p62, and this interaction is dependent upon the SH3 domain of Fyn(5). Studies on mice expressing kinase-inactive Fyn and on Fyn knockout mice indicate that Fyn is likely to play a role in the later stages of thymocyte development, but is not required for TCR- mediated proliferation in peripheral cells(6,7,8). Applications: Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: FLISA: 0.1-1ug/ml Immunoprecipitation, IP-Kinase Assay: 2-5ug Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice