F9500-03C

Fyn (FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn)

Size

£642.00

SKU:: F9500-03C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: VQCKDKEATKLTE, N Terminus
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Alternate Name: FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn Domain Information: Eukaryotic protein kinase domain; SH3 domain; Serine/Threonine protein kinases, catalytic domain; Src homology 2 domains; Src homology 3 domains; Src homology domain 2; Tyrosine kinase, catalytic domain Gene Ontology Terms: learning; feeding behavior; protein kinase cascade; protein phosphorylation; protein tyrosine kinase Application(s): Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55-60kD band observed in Jurkat, A431 and NIH-3T3 lysates (predicted MW of 62kD according to NP_002028.1). Recommended for use at 0.2-1ug/ml. Staining 0.2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See F9500-03C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Shipping Conditions:: Blue Ice