F9200-52
Fucosyltransferase 11 (FUT11, FucTXI, alpha 1,3 fucosyltransferase 11)
Size
£825.00
- SKU:
- F9200-52
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- CHO cell-derived recombinant human Fucosyltransferase 11 (aa25-492).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
- Extra Details:
- FUT11 (Fucosyltransferase 11; also FucTXI and alpha 1,3 fucosyltransferase 11) is a 56kD (predicted) member of the glycosyltransferase 10 family of enzymes. It is potentially an ER associated enzyme that catalyzes the addition of alpha-L-fucose to a biantennary N-glycan acceptor. Rather than serve as a permanent modification, this fucosylation may serve as a quality control mechanism, flagging inappropriately glycosylated molecules. Human FUT11 is a type II transmembrane protein 492aa in length. It contains a short cytoplasmic region (aa1-7) plus an extended lumenal domain (aa25-492). that possesses a transferase region (aa60-360). There are two potential splice variants. One shows an Arg sunstitution for aa445-472, while a second shows a 31aa substitution for aa446-472. Over aa25-492, human FUT11 shares 88% aa identity with mouse FUT11. Applications: Suitable for use in ELISA, Immunoprecicpitation, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice