FOXD3 (Forkhead Box D3, HFH2, HNF-3 Forkhead Transcription Factor Genesis)

Size

£863.00

SKU:: F9046-10F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant corresponding to aa1-140 from human FoxD3 expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: FOXD3 is a 58-60kD member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and neural progenitors of the developing spinal cord. FOXD3 is believed to maintain an uncommitted state in multipotent neural crest stem cells, and to induce cell cycle arrest via p53 and p21cip1. Human FOXD3 is 478aa in length. It contains a forkhead DNA binding domain aa141-235 plus an aa262-473. Over aa1-140 and aa302-462, human FOXD3 shares 76% and 91% aa identity with mouse FoxD3. Applications: Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.