F7080-01

Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)

Size

£825.00

SKU:: F7080-01
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Asp75-Ile376 from human Fibromodulin, expressed in NS0 cells. Species Sequence Homology: mouse and rat, 96%
Clone:: 9L829
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50-70kD protein contains a sulfated N-terminal complement C1q binding domain (aa19-98) and 12 leucine-rich repeats (aa75-376). A 40kD form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa identity with mouse or rat FMOD. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Direct Western Blot: 2ug/ml using human cartilage tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice