F7000-05C

FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)

Size

£889.00

SKU:: F7000-05C
Purity:: Purified by Immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Asn121-Asn449 from human FRS2 expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: FRS2 (FGFR substrate 2; also known as SNT and FRS2-Alpha) is a 70-90kD member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids (aa) in length. It contains a membrane-anchoring myristoylation signal (aa 1-6), a PTB domain that interacts with FGF and NGF receptors (aa 13-115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196-471). One splice form shows a deletion of the C-terminal four amino acids. Over aa 121-449, human FRS2 is 99% and 94% aa identical to canine and mouse FRS2, respectively. Applications: Suitable for use in Western Blot, Immunohistochemistry and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human liver subjected to antigen retrieval. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice