F6401-37B
Frizzled 8 (FZD8) (Biotin)
Size
£876.00
- SKU:
- F6401-37B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived recombinant mouse Frizzled-8 extracellular Cysteine-Rich Domain (CRD).
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.2, with BSA. Reconstitute with 1ml of TBS, 0.1% BSA. Labeled with Biotin.
- Extra Details:
- Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant mouse Frizzled-8 is ~5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute with sterile Tris-buffered saline, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice