F6401-37A
Frizzled 8 (FZD8)
Size
£557.00
- SKU:
- F6401-37A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NS0-derived rmFrizzled-8 extracellular domain (CRD)
- Clone:
- 9j131
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: Use at 1-2ug/ml. Using a colorimetric detection system, the detection limit for rmFrizzled-8 is ~25ng/lane under reducing conditions. Direct ELISA: Use at 0.5-1.0ug/ml. The detection limit is ~5ng/well. Optimal dilutions to be determined by the researcher. Endotoxin: ≤1Eu/ug Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice