Frizzled 5 (FZD5)

Size

£889.00

SKU:: F6401-17
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: NS0-derived, recombinant protein corresponding to Ala27-Pro167 from human Frizzled 5 extracellular Cysteine-Rich Domain (CRD)
Format:: Supplied as a liquid in PBS, pH 7.2, 5% trehalose.
Extra Details:: Frizzled 5 belongs to the Frizzled family of integral membrane proteins that are receptors for Wnt proteins. Frizzled receptors have seven transmembrane domains and a long N-terminal cysteine-rich domain that bind directly to the Wnt proteins. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1ug/ml. The detection limit for rhFrizzled 5 is ~0.5ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit for rhFrizzled 5 is ~25ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.