Frizzled 4 (Frizzled-4, Frizzled (Drosophila) Homolog 4, Fz 4, Fz-4, FZD4, FZD4S, FzE4, CD344 Antigen, EVR1, FEVR, GPCR, hFz4, WNT Receptor Frizzled-4, Exudative Vitreoretinopathy 1 Autosomal Dominant Criswick-Schepens Syndrome) (Biotin)

Size

£863.00

SKU:: F6401-15C
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: NS0-derived rmFrizzled-4 extracellular Cysteine-Rich Domain (CRD)
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Extra Details:: Application: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-4. The detection limit for rmFrizzled-4 is ~5ng/lane under non-reducing and reducing conditions. In this format, this antibody shows less than 2% crossreactivity with rmFrizzled-3, rmFrizzled-7 and rmFrizzled-8. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.