F6401-15B

Frizzled 4 (Frizzled-4, Frizzled (Drosophila) Homolog 4, Fz 4, Fz-4, FZD4, FZD4S, FzE4, CD344 Antigen, EVR1, FEVR, GPCR, hFz4, MGC34390, WNT Receptor Frizzled-4, Exudative Vitreoretinopathy 1 Autosomal Dominant Criswick-Schepens Syndrome) (Biotin)

Size

£780.00

SKU:: F6401-15B
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: <0.15EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Met1-Glu180 from mouse Frizzled-4 expressed in NS0 cells.
Clone:: 9j127
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.5% BSA. Labeled with Biotin. Reconstitute with 1ml sterile PBS.
Extra Details:: Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.25ug labels 10e6 cells using HEK293 human embryonic kidney cell line Immunocytochemistry: 8-25ug/ml using immersion fixed HEK293 human embryonic kidney cell line Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.