F6401-15A

Frizzled 4 (Frizzled-4, Frizzled (Drosophila) Homolog 4, Fz 4, Fz-4, FZD4, FZD4S, FzE4, CD344 Antigen, EVR1, FEVR, GPCR, hFz4, WNT Receptor Frizzled-4, Exudative Vitreoretinopathy 1 Autosomal Dominant Criswick-Schepens Syndrome)

Size

£563.00

SKU:: F6401-15A
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Phe37-Glu180 of mouse Frizzled-4 expressed in NS0 cells.
Clone:: 9j127
Format:: Supplied as a liquid in PBS, pH 7.4, 5% trehalose.
Extra Details:: Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. Frizzled-4 is expressed by a variety of tissues. It is implicated in several developmental processes, notably in retinal angiogenesis. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. HEK293 human embryonic kidney cell line. Immunocytochemistry: 8-25ug/ml in immersion fixed HEK293 human embryonic kidney cell line. Western Blot:1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: NS0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.