F6401-11D
Frizzled 3 (FZD3) (Biotin)
Size
£780.00
- SKU:
- F6401-11D
- Purity:
- Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse Frizzled 3 extracellular Cystine Rich Domain, expressed in NS0 cells.
- Clone:
- 9j126
- Format:
- Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Frizzled 3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit for recombinant mouse Frizzled-3 is ~0.5ng/lane under non-reducing and reducing conditions. Flow Cytometry: 0.2mg/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: 8ug/ml detects Frizzled 3 in human HEK 293 cells or frozen mouse embryos. Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, recombinant mouse Frizzled 3 extracellular Cysteine-Rich Domain (CRD) expressed in NS0 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice