F6401-11C
Frizzled 3 (FZD3)
Size
£850.00
- SKU:
- F6401-11C
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: <0.1EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to the extracellular CRD domain of mouse Frizzled 3 expressed in NS0 cells.
- Clone:
- 9j126
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins. Applications: Suitable for use in ELISA (direct), Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended DIlutions: Flow Cytometry: 5-10ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as a goat anti-rat IgG (FITC). Immunohistochemistry: A biotin conjugate of this antibody (F6401-11D) was used at a concentration of 8ug/ml to detect Frizzled 3 in frozen mouse embryo sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice