F6401-07B
Frizzled 2 (FZD2) (Biotin)
Size
£863.00
- SKU:
- F6401-07B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived rmFrizzled-2 extracellular cysteine-rich domain (CRD)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Frizzled-2 belongs to the Frizzled family of 7-pass transmembrane receptors for Wnt proteins. Frizzled-2 is most extensively studied for its role in cardiac development and neural crest differentiation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Use at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-2. The detection limit for rmFrizzled 2 is ~10ng/lane and 2ng/lane under non-reducing and reducing conditions. In this format, this antibody shows ~25% crossreactivity with rmFrizzled 1 and less than 5% crossreactivity with rmFrizzled 3, rmFrizzled 4, rmFrizzled 5, rmFrizzled 6, rmFrizzled 7 and rmFrizzled 8. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice